Table 1.
Mice with reporters in the endogenous genes
| Donor | Blastocysts/Injected zygotes | Targeted blastocysts/Total | Targeted ESCs/Total | Transferred embryos (recipients) | Knock-in pre- and postnatal mice/Total | |
|---|---|---|---|---|---|---|
| Tet1-loxP + Tet2-loxP | 65/89 | Tex1-loxP | 6/15 | N/A | N/A | N/A |
| Tex2-loxP | 6/15 | |||||
| Both | 3/15 | |||||
|
| ||||||
| Sox2-V5 | 414/498 | ND | 7/16 | 200(10) | 12/35 | |
|
| ||||||
| Nanog-mCherry | 936/1262 | 86/936 | ND | 415 (21) | 7/86 | |
|
| ||||||
| Oct4-GFP | 254/345 | 47/254 | 3/9 | 100(4) | 3/10 | |
Cas9 mRNA, sgRNAs targeting Tet1, Tet2, Sox2, Nanog, or Oct4, and single stranded DNA oligos or double stranded donor vectors were injected into fertilized eggs. Targeted blastocysts were identified by RFLP or fluorescence of reporters. The blastocysts derived from injected embryos were derived ES cell lines or transplanted into foster mothers and E13.5 embryos and postnatal mice were obtained and genotyped.
ND, not determined.