Figure 7.
VEGF-B induced metabolic changes in the heart.
- Venn-diagram and functional clustering of genes downregulated by VEGF-B (N = 6 + 6 in both experiments). Functional clustering showed that most of the forty genes downregulated in both TG and AAV-VEGF-B hearts are related to fatty acid metabolism.
- Heat map from the GSEA analysis, showing significant downregulation of genes related to fatty acid degradation in both AAV-VEGF-B and VEGF-B TG hearts.
- Malonyl-CoA content was significantly increased in TG hearts. *P = 0.005
- AMPK phosphorylation (*P = 0.019) and perilipin 5 expression (***P = 3.8 × 10−5) were significantly reduced in TG hearts, whereas the expression of fatty acid synthase (FASN) was increased (*P = 0.022). There was no change in pyruvate dehydrogenase kinase 4 (PDK4), PGC-1α or cytochrome c (CytC). Fold change (FC) shows the relative expression in the TG hearts compared to the WT hearts (N = 6 + 6).
- Triglyceride content was reduced in the TG hearts (**P = 0.007) but not changed in the KO hearts. Expression of metabolic genes in TG (F) and KO (G) hearts compared to their respective controls.
Data information: Data are shown as mean ± s.e.m. (Student's t-test). This figure is accompanied by supplementary Fig 6.