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V. cholerae colonization of zebrafish at earlier time points. Zebrafish were exposed to either 3 × 1010 V. cholerae classical strain O395 (A) or 3 × 109 V. cholerae El Tor strain E7946 (B) cells. At the indicated time points, fish were sacrificed and intestinal V. cholerae levels were determined by plating of serial dilutions of the intestinal homogenates.
