Figure 5. F15R-Kit cell size increases with cytokine dose but proliferation remains identical.
(A) Median FSC values for F15R-Kit cells cultured for 48h with the indicated doses of IL-2 or IL-15 normalized to the 100pM dose for each cytokine. *αfw < 0.05, **αfw < 0.01, ***αfw < 0.001. (B) Plot showing the ratios of the CFSE values of F15R-Kit cells cultured with the indicated dose of cytokine to the unstimulated cells at 48h. ***αfw < 0.001 compared to unstimulated cells. (C) Representative flow cytometry dot plots showing forward scatter and histograms showing CFSE levels of F15R-Kit cells 48h after treatment with the indicated dose of IL-2 or IL-15. Dotted histograms represent unstimulated cells. Data represent Mean ± SD for 3 independent replicates.
