TABLE 2.
Comparison of aa residues demonstrated (Upf1 and PcrA) or predicted (ToMV-Hel and N470) to interact with different moieties of the NTP molecule, incoming water molecules, and Mg2+ cations
The numbers of corresponding conserved motifs are shown in parentheses.
| Interacting partner | Upf1 (45) | PcrA (26) | ToMV-Hel (44) | CHIKV N470 (this study) |
|---|---|---|---|---|
| Mg2+ | Asp-636 (II) | Asp-223 (II) | Asp-906 (II) | Asp-252 (II) |
| Water molecule | Glu-637 (II) | Glu-224 (II) | Glu-907 (II) | Glu-253 (II) |
| Nucleobase pocket | Tyr-702 (IV) | Tyr-286 (IV) | Leu-967 (IV) | Arg-311 (IV) |
| Ribose ring | Val-500 (I) | Arg-39 (I) | Lys-841 (I) | Ala-194 (I) |
| γ-Phosphate | Lys-498 (I) | Lys-37a (I) | Lys-839 (I) | Lys-192 (I) |
| Gln-665 (III) | Gln-254 (III) | Gln-936 (III) | Gln-283 (III) | |
| Arg-703 (IV) | Arg-287 (IV) | Arg-968 (IV) | Arg-312 (IV) | |
| Arg-865 (VI) | Arg-610 (VI) | Arg-1076 (VI) | Arg-416 (VI) |
a Interacts with β-phosphate.