Figure 4.
Effect of c-jun knockdown on combination MEKi + PI3Ki treatment of uveal melanoma cells. A, GNAQ Q209L mutant uveal melanoma cells (92.1) were treated with combination MEKi (10nM) + PI3Ki (100nM) for 4 hours and levels of phosphorylated c-jun determined relative to untreated cells. B, Cells were transfected with two distinct siRNAs targeting c-jun, then levels of phosphorylated c-jun, total c-jun or beta-actin were determined by western blot. C, Cells were transfected with two distinct siRNAs targeting c-jun, then treated with combination MEKi (10nM) + PI3Ki (100nM). The effect of c-jun knockdown in MEKi + PI3Ki treated cells was compared to the effect of MEKi + PI3Ki treatment alone. Asterisks (*) indicate p value <0.05.