Figure 8. TGFβ neutralizing antibody reduces pSmad2 and FN mRNA up-regulation on FN.
(A) 10 μg/ml of TGFβ neutralizing antibody with or without 0.05 ng/ml TGFβ1 were added to MCF-10A cells plated on MG or FN without serum. Cell lysates were prepared after 2 hr and used for immunoblotting with pSmad2, total Smad, or GAPDH antibodies. (B) qRT-PCR was used to measure mRNA levels for FN in cells grown on FN or MG for 6 hr without serum and treated as in (A). At least three independent experiments were averaged. Values were normalized to the level in untreated cells on MG. * p < 0.05 between the columns.