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. Author manuscript; available in PMC: 2014 Feb 20.
Published in final edited form as: Cell. 2011 Jun 10;145(6):926–940. doi: 10.1016/j.cell.2011.04.029

Figure 1. A mesenchymal subpopulation (MSP) isolated from immortalized human mammary epithelial cells (HMLE) cells.

Figure 1

(A) Bright phase microscopy: MACS-purified HMLE24+, HMLE overexpressing Twist (HTwist) and re-attached MSP cells. (B) Immunofluorescence: adherens junction protein E-cadherin and tight junction protein ZO-1 in HMLE24+, HTwist and MSP cells. (C) Immunofluorescence: mesenchymal intermediary filament vimentin and epithelial cytokeratins (panCK = pan-cytokeratin antibody), expression of fibronectin and Twist. (D) Immunoblot: E- and N-cadherin, EMT-TFs ZEB1, Snail and Slug, loading control: β-actin. (E) Flow cytometry: CD44-APC and CD24-PE cell-surface staining. Numbers indicate % of CD44hi/CD24− population. (F) Mammosphere Assay: bright phase microscopy images and quantification of parental HMLE, HMLE24+, HTwist and MSP as well as FACS-purified CD44hi/CD24− and CD44−med(ium)/CD24+ HMLE cells; n=12. (G) Migration assay: microscopy images and quantification; n=3.