Figure 3. Poly I:C inhibition of fibrotic gene expression is TLR3-independent.
A. Fibroblasts were pre-treated with chloroquine (10 µM) for 60 min, followed by Poly I:C (10 µg/ml) for a further 24 h, and RNA was analyzed by real-time qPCR. The results represent the means ± SEM of triplicate determinations. B, C. Fibroblasts were transiently transfected with indicated siRNA or scrambled control siRNA (siCTR) in parallel, and incubated in media with or without Poly I:C (10 µg/ml) for a further 24 h, followed by real-time qPCR. Results are the means ± SEM of triplicate determinations. *p<0.05, **p<0.001