Figure 4. Motion of bead cargo in association with MT disassembly.
(a) Schematics of the segmented MT assay in vitro using bead cargo. (b, c) A cartoon and still images from segmented MT assay with FL (panels b) and TR CENP-E-coated beads (panels c). Bead (yellow arrowhead) was placed on a capped MT grown from a coverslip-attached axoneme, as drawn in the left panel. Fluorescently-labeled cap (white arrowhead) was photo-ablated via the Rhodamine excitation (red color). All other images were recorded with DIC illumination. Numbers above panels are seconds from the start of bead's walking. (d) Sample motions of the CENP-E-coated beads. Distance is from the minus MT end. Arrowheads indicate bead detachment from the MT. FL CENP-E-coated beads sometimes remained attached even after MT has stopped shortening. (e) Tip-tracking frequency of FL (n=10) and TR CENP-E coated beads (n=18). Error bars are SEM estimated by bootstrapping (n=1,000 samples).