FIGURE 3.
IL-4-dependent gene expression. A, Expression of IL-4 and LT-α mRNAs by γδ T cells purified from PBMC after 72 h, as analyzed by real-time RT-PCR. Expression levels were normalized to values in medium controls and represent mean values and SDs from duplicate experiments. B, Expression of IL-4 and GATA-3 mRNAs by γδ T cells purified from PBMC after 72 h. Semiquantitative RT-PCR data shown are representative of three blood donors. C, Surface expression of CD27 (n = 6–7) as analyzed by flow cytometry on day 6.