Fig. 3.
A. Grm1-receptor ectopically expressed in W2 cells induces tumorigenicity in independent clones (W2-Grm1). Average tumor size upon termination at 13 weeks after inoculation with 106 cells (n=20). No measurable tumors were ever observed from empty-vector transfected cells (W2-vec) at this endpoint. Representative nude-mouse/tumor photographs, and photomicrograph of typical H&E stained tumor section with blood vessel. Immunoblotting demonstrated Grm1 expression in tissue excised from Grm1-iBMK tumors.
B. Grm1-receptor ectopically expressed in D3 cells increases tumorigenicity in independent clones (D3-Grm1). At the endpoint, 8 weeks after inoculation with 106 cells (n=20), Grm1-receptor expressing clones reached a mean tumor size over 1000 mm3, whereas D3-vec cell tumors only averaged approximately one-fifth that volume (P < 0.001, ANOVA). Representative nude-mouse/tumor photographs include closer view of tumor angiogenesis visible through the skin as overt blood vessels, in addition to photomicrograph of H&E stained section. Immunoblotting demonstrated Grm1 expression only in tissue from Grm1-iBMK seeded tumors.