Table 2.
X-Ray Data Collection Statistics
| SeMet BpsDsbA L83M L132M | Wild-type BpsDsbA | |
|---|---|---|
| Data collection | ||
| Wavelength (Å) | 0.97930 | 0.95370 |
| Resolution (Å) | 68.3–2.50 | 46.2–1.9 |
| Spacegroup | P212121 | P212121 |
| Unit cell dimensions (Å,°) | a=59.69, b=61.44 | a=59.32, b=62.30 |
| c=68.33 | c=68.78 | |
| α=β=γ=90.0 | α=β=γ=90.0 | |
| Observed reflections | 250265 | 140971 |
| Unique reflections | 9208 | 20726 |
| Rmerge | 0.142 (0.725) | 0.102 (0.732) |
| Rmeas | 0.147 (0.751) | 0.111 (0.79) |
| Rpim | 0.030 (0.143) | 0.043 (0.296) |
| Completeness (%) | 100 (100) | 100 (100) |
| I/σI | 23.6 (5.9) | 10.1 (2.6) |
| Multiplicity | 27.2 (27.6) | 6.8 (7.0) |
| SAD phasing | ||
| Resolution (Å) | 68.3–2.50 | – |
| Number of selenium atoms found/expected | 3/3 | – |
| Mean figure of merit | 0.36 | – |
Values in parentheses refer to the highest resolution shell.
SAD, single-wavelength anomalous dispersion.