FIG. 3.
Deletion of dsbA attenuates virulence in vitro and in vivo. (A) Intracellular survival of Burkholderia pseudomallei WT (white bars) and ΔdsbA (black bars) in J774A.1 macrophages. Mean values are plotted from three experiments with SEM. (B) ΔdsbA is significantly attenuated compared with WT in a murine model of infection [log-rank (Mantel–Cox) test p<0.001]. Groups of six BALB/c mice were infected by the intraperitoneal (i.p.) route with B. pseudomallei strains: WT 3.43×104 CFU (▲), ΔdsbA 5.77×104 CFU (■).WT, wild type; CFU, colony forming unit.