Figure 3.
Measurement of acetylated Egr-1 in primary MAECs and aortic tissues from apoE−/−hAR mice using immunoprecipitation (IP) with anti-acetyllysine antibody followed by Western blotting for Egr-1. A: Expression of acetylated Egr-1 in MAECs overexpressing hAR in cells treated with high glucose and 5.5 mmol/L glucose for 24 h along with ARI and SDI. B: Time-dependent expression of acetylated Egr-1 in MAECs treated with high glucose. C: Expression of acetylated Egr-1 in HAECs treated with 5.5 mmol/L and high glucose along with ARI and SDI. D: Acetylated Egr-1 expression in aorta isolated from 14-week diabetic (DM) and nondiabetic (NDM) apoE−/−hAR mice and in DM mice treated with ARI for 14 weeks. Results are representative of at least three independent experiments. Ac-lysine, acetyllysine; WB, Western blot.