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. Author manuscript; available in PMC: 2014 Jan 20.
Published in final edited form as: ACS Nano. 2011 Mar 2;5(4):2756–2769. doi: 10.1021/nn200328m

Figure 8. Confocal microscopy to show the co-localization of syndecan-1 with FITC-labeled MSNP-PEI in differentiated cells.

Figure 8

Syndecan-1 was detected by an antibody coupled to Alexa Fluor 594 (red fluorescence). FITC-labeled MSNP-PEI 10 kD (25 μg/mL) was added to the cells for 3 hr prior to protein and nuclear staining as in Fig. 7A. The combined panel on the right hand side demonstrates particle co-localizing with syndecan-1. The extent of the co-localization was 64.3% as determined by Image J software.