Figure 3.
Inhibitory effect of telomestatin (TMS) on GSC migration. A, representative pictures of human-specific nestin staining of immunocompromised mouse brains bearing human GBM157 sphere–derived tumors (top). Original magnification, 10×. *, location of xenograft. The rectangle indicates the region of magnification in the bottom. Human-specific nestin staining indicates migrated human GBM cells to the contralateral side of mouse brains through the corpus callosum in the control (DMSO-treated) group (bottom left) and telomestatin-treated group (bottom right). Original magnification, 40×. B, experimental flow for the migration assay in organotypic cultures of mouse brain slices. C, graphs for the time course of migration of GBM spheres (GSCs) and serum-propagated cells (non-GSCs) derived from 2 GBM samples (GBM146 and 157). Quantitative analysis indicates cell dispersion (Area tx/Area t0). ***, statistical significance by 2-way ANOVA for repeated measures (P < 0.001). D, representative pictures of GFP-expressing human GBM146 cells dispersing on brain slices after treatment with 1 µmol/L of telomestatin or DMSO (left). Graphs indicate the relative dispersion of the cells normalized the initial size and fluorescence of the spheres (right). *, statistical significance by 2-way ANOVA for repeated measures (**, P < 0.05; ***, P < 0.001).