FIGURE 2.
Role of CD14 in TLR4 internalization in corneal epithelial cells. A, constitutive expression of CD14 on the surface of HCEC and HCET human corneal epithelial cell lines analyzed by flow cytometry after 4-h incubation with LPS and IFN-γ. B, reduction in cell surface TLR4 after 15-min, 30-min, and 1-h incubation with LPS together with either control IgG or blocking anti-CD14. Left panels show representative profiles, and right panels show time course as a percentage of initial mean fluorescence intensity (MFI). C, intracellular TLR4 under the same conditions. These experiments were repeated three times with similar results.