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. 1974 Jan;71(1):57–62. doi: 10.1073/pnas.71.1.57

Characterization of a New Murine Cellular DNA Polymerase

D M Livingston 1,2,*, L E Serxner 1,2, D J Howk 1,2, J Hudson 1,2, G J Todaro 1,2
PMCID: PMC387931  PMID: 4129804

Abstract

A new DNA polymerase (peak A) has been identified in the high-speed pellet fraction of two subclones of nonvirus producing Balb/3T3 cells. The activity is associated with a molecule of approximately 70,000 molecular weight and chromatographs in two systems like the mouse type-C viral reverse transcriptase and a similar enzyme from the high-speed pellet fraction of a virus producing Balb/3T3 subclone, S2Cl3. Comparable quantities of peak A are present in both nonvirus infected (A31) and mouse sarcoma virus transformed nonproducer (KA31) subclones. Virus producing cells contain 10-20 times more peak A polymerase activity. A31 and KA31 peak A are comparably inhibited by anti-mouse type C virus reverse transcriptase IgG but to a lesser degree than S2Cl3 peak A or authentic viral reverse transcriptase. They can also be differentiated from the latter two enzymes by template preference studies. KA31 peak A can be distinguished from three other KA31 DNA polymerases (R-DNA polymerase and DNA polymerase N and C), and thus appears to be a new species of cellular DNA polymerase.

Keywords: poly(A) directed poly(dT) synthesis, type-C viral reverse transcriptase, R-DNA polymerase

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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