FIG. 6.
Transgene expression by chimeric Ad5/F3 and CPE of wild-type Ad3 in BHK-CD46 cells. (A) Ad-mediated eGFP expression in permissive human cells and CD46- or CAR-transfected rodent cells. The indicated cells were incubated with eGFP-expressing Ad5 or Ad5/F3 at different MOIs, and eGFP expression was analyzed at 2 days p.i. by flow cytometry. Results are shown as the mean fluorescence intensity (MFI). (B) CPE of wild-type Ad3 in CD46-transfected BHK cells and human HeLa cells and control infections of BHK-CD46 and HeLa cells with eGFP-expressing Ad5. Cells were infected with 10-fold dilutions of virus (3 × 101 to 3 × 104 particles/cell) for 3 days, fixed with methanol, and stained with crystal violet.