TABLE 2.
Description of SARS PCR assaysa
| Assay | Amplification target | Product size (bp) | RT-PCR type | Assay format | RT-PCR vol (μl) | PCR vol (μl) | No. of amplification cycles |
|---|---|---|---|---|---|---|---|
| 1 | PolB | 190 (1st), 109 (2nd) | One step | CHB nested | 2 (1st), 1 (2nd) | 20 (1st), 50 (2nd) | 50 (1st), 35 (2nd) |
| 2 | PolB | 190 | Two step | CHB | 5 (RT), 4 (PCR) | 20 (RT), 50 (PCR) | 50 |
| 3 | PolB | 368 | Two step | CHB | 5 (RT), 4 (PCR) | 20 (RT), 50 (PCR) | 50 |
| 4 | PolB | 190 | One step | LC (SYBR Green) | 2 | 20 | 50 |
| 5 | Nucleocapsid | 149 | Two step | LC (SYBR Green) | 5 (RT), 4 (PCR) | 20 (RT), 50 (PCR) | 50 |
| 6 | Nucleocapsid | 149 | One step | LC (TaqMan probe) | 5 | 20 | 50 |
| 7 | NAb | 80 | One step | LC (TaqMan probe) | 5 | 20 | 50 |
a
1st, first round; 2nd, second round.
b
NA, not available from the manufacturer (Artus).