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. Author manuscript; available in PMC: 2013 Dec 17.
Published in final edited form as: Nat Commun. 2013;4:10.1038/ncomms3267. doi: 10.1038/ncomms3267

Figure 2. HLH-30 is required for TOR inhibition to extend C. elegans lifespan.

Figure 2

(a) Nuclear localization of HLH-30 was quantified in day 1 adult animals expressing HLH-30::GFP. Animals were fed bacteria expressing control or tor dsRNA from hatching and raised at 20 °C (three biological replicates, ~50 animals each, mean±s.d., **P<0.01, Student’s t-test). (b, c) Expression of putative autophagy-related and lysosomal target genes was measured by quantitative PCR in day 1 (b) WT (N2) and (c) hlh-30(tm1978) animals fed bacteria expressing control or tor dsRNA from hatching (20 °C). Data are mean±s.d. of biological triplicates. *P<0.05, **P<0.01; Student’s t-test. (d) Lifespan analysis of WT animals and hlh-30(tm1978) mutants fed bacteria expressing control or tor dsRNA from day 1 of adulthood was carried out at 20 °C. See Supplementary Table S3 for details of lifespan analyses and replicate experiments.