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. Author manuscript; available in PMC: 2013 Dec 15.
Published in final edited form as: Biochem J. 2012 Nov 15;448(1):10.1042/BJ20121110. doi: 10.1042/BJ20121110

Figure 1.

Figure 1

Palmitate, palmitoyl-CoA (P-CoA) and palmitoyl carnitine (PC) respiration kinetics in permeabilized muscle fibres. Palmitate was titrated in the presence of malate (2mM), ADP (5mM), Co-enzyme A (1mM) and L-carnitine (2mM). P-CoA was titrated in the presence of malate (2mM), ADP (5mM) and L-carnitine (2mM). PC was titrated in the presence of malate (2mM) and ADP (5mM). All respiration chambers contained 25 μM blebbistatin. All R2 values are above 0.98. P-CoA was inhibitory on respiration at ∼220 μM and PC was inhibitory on respiration at 100 μM. N=6 and each independent experiment was performed in duplicate, and data is expressed as mean ± SEM.