Skip to main content
. Author manuscript; available in PMC: 2014 Nov 11.
Published in final edited form as: Dev Cell. 2013 Nov 11;27(3):10.1016/j.devcel.2013.10.008. doi: 10.1016/j.devcel.2013.10.008

Figure 5. Shh signaling supports proliferation and expansion of GABAergic interneuron progenitors in PWM domains.

Figure 5

(A–L) Analysis of TncYFP-CreER and TncYFP-CreER; SmoF/− cerebella from mice injected with TM on P1, P2 and analyzed at P3 (A, B), P5 (C–F), P7 (G–J) or P30 (K, L) with either the Gli1nlacZ (AH) or Ai9F/+ (I–L) reporter. Gli1 expression as detected by X-gal is reduced in PWM of P3 TncYFP-CreER; SmoF/− Gli1nlacZ; SmoF/− cerebella when compared to the control TncYFP-CreER; Gli1nlacZ (A, B). Similarly, Brdu+ Sox2+ (C–D′), Ptf1a+ (E, F), Pax2+ (G, H, I, J) and Parv+ (K, L) cells are also significantly reduced in the mutants. (M–Q) Quantitative analyses of BrdU+, Sox2+ cells over total Sox2+ cells (M), Ptf1a+ cells (N), Pax2+ cells (O), total Pax2+ cells (P) and Parv+ cells (Q) in TncYFP-CreER (grey bar) and TncYFP-CreER; SmoF/− (open bar) cerebella. All the analyses were performed on 5 to 10 sections per brain and with three or four animals per genotype. Error bars are presented as SEM, and p value relative to control samples indicated. White dotted lines demarcate the PWM, white arrowheads indicate double-labeled cells. Scale bars indicate 25 μm. See also Figure S5.