Table 1.
PCR primers used in the study
| Gene | MGI Symbol | Forward primer | Reverse primer |
|---|---|---|---|
| TNF | Tnf | TTGGAGTCATTGCTCTGTGAA | GGGTCAGAGTAAAGGGGTCAG |
| MCP-1 | Ccl2 | AGGTGTCCCAAAGAAGCTGTA | ATGTCTGGACCCATTCCTTCT |
| IL-6 | Il6 | ATGGATGCTACCAAACTGGAT | TGAAGGACTCTGGCTTTGTCT |
| IL-1β | Il1b | CTCTCCACCTCAATGGACAGA | TTTTGTCGTTGCTTGGTTCTC |
| ICAM-1 | Icam1 | GCCTTGGTAGAGGTGACTGAG | GACCGGAGCTGAAAAGTTGTA |
| ELAM-1 | Sele | CTCACTCCTGACATCGTCCTC | ACGTTGTAAGAAGGCACATGG |
| TGF-β1 | Tgfb1 | GAGAGCCCTGGATACCAACTA | GACAGAAGTTGGCATGGTAGC |
| TGF-β2 | Tgfb2 | TGGCCGAGCAGCGGATTGAAC | TGCACAGCGTCTGTCACGTCG |
| TGF-β3 | Tgfb3 | TGCTTCCGCAACCTGGAGGAGA | CTGCGCTGCGGAGGTATGGG |
| RANTES | Ccl5 | ATATGGCTCGGACACCACTC | GTGACAAACACGACTGCAAGA |
| SLPI | Slpi | GTGACGGCAAATACAAGTGCT | CTGGGAGCAGGGAAGTAGTTT |
| INOS | Nos2 | CAGCTGGGCTGTACAAACCTT | CATTGGAAGTGAAGCGTTTCG |
| NOX2 | Cybb | CCAACTGGGATAACGAGTTCA | GAGAGTTTCAGCCAAGGCTTC |
| COX-2 | COX2 | TGGTGCCTGGTCTGATGATG | GTGGTAACCGCTCAGGTGTTG |
| MMP-2 | Mmp2 | GAATGCCATCCCTGATAACCT | CAGCCAGTCTGATTTGATGCT |
| MMP-9 | Mmp9 | ATTCGCGTGGATAAGGAGTTC | CCTTGTTCACCTCATTTTGGA |
| CLDN5 | Cldn5 | TGGTGGCACTCTTTGTTACCT | AGTGCTACCCGTGCCTTAACT |
| VE-CADHERIN | Cdh5 | CTGTGGAGACCATAGGTGACAG | CTGGGATCTTTCTGGTGAGTG |
| PDGFB | Pdgfb | CGGCCGGTCCAGGTGAGAAAG | CACTTGCAGGCGAGGTGGTCC |
| PDGFRα | Pdgfra | CTCCTCACGCTGTGTGCGACC | GGCGGGATCCATCCAAGCCAT |
| PDGFRβ | Pdgfrb | ACTGCCGTGCAGCCCAATGAG | GGTGGAACTGGCAAGGCTGGG |
| VEGFA | Vegfa | CCAGGAGGACCTTGTGTGAT | GAGGAAGGGTAAGCCACTCAC |
| VEGFR1 | Ft2 | AGGAAGACGGTTCCTTCTGAG | CCCAGTGCACCTAGGTATGTC |
| VEGFR2 | Kdr | GGGGAACTGAAGACAGGCTAC | CGGCCAAGAGGTTTTCCTAGT |
| ANG1 | Angpt1 | GCTTCCCACCACATCTTTCTT | CCTGTAATGTCGGCACATACC |
| ANG2 | Angpt2 | ACTGGGAAGGCAACGAGGCG | GCGGTCCCCGTGAGTCCTGTAA |
| TIE2 | Tek | GGAAAATTTAACCCCATCTGC | CAGTGAATATGGCCACTGAGAA |
| tPA | Plat | AATAAAACCGTCACGAACAACA | TTTATTGATCATGCACACCAGAG |
| AQP4 | Aqp4 | TACATGGAGGTGGAGGACAAC | TCTTCTCTTCTCCACGGTCAA |
| HPRT | Hprt | CCGAGGATTTGGAAAAAGTGT | GGAAAATACAGCCAACACTGC |
SLPI, secretory leukocyte protease inhibitor; COX-2, cyclooxygenase-2; CLDN5, claudin-5; MGI, mouse gene identifiers.