Figure 4. Knockdown of C2ORF40 promotes breast cancer cell growth and migration-invasion. (A) Knockdown of C2ORF40 in SKBR3 and MDAMB468 cells as indicated by RT-PCR (upper panel) and western blotting (lower panel). Knockdown of C2ORF40 promotes SKBR3 and MDAMB468 cell proliferation assessed by MTT method (B, C). Representative photographs show knockdown of C2ORF40 promotes migration of SKBR3 (D) and MDAMB468 (E) cells in a scratch-wound healing model on cultured cells. Knockdown of C2ORF40 promotes the invasive ability of SKBR3 (F) and MDAMB468 (G) cells using Matrigel assay. Images in (D) to (G) show representative field of views. All experiments were repeated at least three times. Data were presented as mean ± SD, * p < 0.05 and ** p < 0.01 obtained from Student?s t-test as compared with control groups. shCtrl: scrambled vector infected control groups.