Figure 1. Reduced C2ORF40 mRNA expression was a significant prognostic factor for disease-free and metastasis-free survival. C2ORF40 expression assessed by Affymetrix microarray in human breast cancers and normal tissues in data set 1 to 3 (Table S1) was shown in panel (A) to (C). The significant association between C2ORF40 mRNA level and disease-free survival was analyzed in four independent cohorts of breast cancer patients (Data set 4 to 7 in Table S1) (D) to (G). The patients from each cohort were divided into groups with high (top one-third), moderate (middle one-third) and low (bottom one-third) level of C2ORF40 expression. Panel (D) to (G) show the Kaplan-Meier survival curves for disease-free survival in the four data sets respectively. Panels (H) and (I) show the Kaplan-Meier survival curves for metastasis-free survival in Data set 4 (NKI) and Data set 7 (GSE6532) respectively. C2ORF40 mRNA is measured as log2 (probe intensities) as in the microarray. The P-values shown were obtained from Mann-Whitney U (A and B), Kruskal-Wallis (C) or long-rank tests (D to I).