Figure 3. IRF4 is dispensable of early T cell activation but vital for CD8+ T cell expansion.
(A) CD25 and CD69 expression of control or IRF4-deficient polycolonal CD8+ T cells (day 1 post activation).
(B) IL-2 in the cultured medium of control or IRF4-deficient polyclonal CD8+ T cells (day 1 post activation). Data are mean ± SD.
(C) Cell numbers of control or IRF4-deficient polyclonal CD8+ T cells following stimulation. Data are mean ± SD.
(D) Cell numbers of OTI T cells stimulated with N4, T4 or Q4H7 peptide. Data are mean ± SD.
(E) 7-AAD staining of control or IRF4-deficient polyclonal CD8+ T cells in the culture.
(F) The percentages of 7-AAD+ cells in control or IRF4-deficient polyclonal CD8+ T cells in the culture (day 2 post activation).
(G) The percentages of active-caspase 3+ cells in the live control or IRF4-deficient polyclonal CD8+ T cells (day 2 post activation).
(H) Proliferation of control or IRF4-deficient polyclonal CD8+ T cells.
(I) The percentages of active-caspase 3+ cells in the live WT or BATF -deficient polyclonal CD8+ T cells (day 2 post activation).
(J) Proliferation of WT or BATF-deficient polyclonal CD8+ T cells.
(K, L) WT CD8+ T cells were left untransduced or transduced with control or IRF4-expressing retroviruses. hCD4 staining (K) and the percentages (L) of hCD4+ cells in live CD8+ T cells at day 2 and day 4 following activation.
(M) OTI T cells were stimulated with N4, T4 or Q4H7 peptide and transduced with control or IRF4-expressing retrovirus. The percentages of hCD4+ cells in live OTI T cells were monitored.
Data are representative of two to four independent experiments except in (F, L). (F, L), data are pooled from four independent experiments. *, P < 0.05. See also Figure S3.