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. Author manuscript; available in PMC: 2014 Oct 15.
Published in final edited form as: J Immunol. 2013 Sep 18;191(8):10.4049/jimmunol.1301170. doi: 10.4049/jimmunol.1301170

FIGURE 2.

FIGURE 2

Activation of NLRP3 inflammasome by MAVS. (A, B) Immunoblots of caspase-1 in cell lysates of 293T-C1A cells transfected with empty vector (Vec) MAVS, NLRP3, NLRP3 plus MAVS full length, or NLRP3 plus MAVS-ΔTM constructs as indicated. (C) Immunoblots of caspase-1 in cell lysates of 293T-C1A cells transfected with empty vector (Vec) or NLRP3 followed by no treatment (Un) or infection with Sendai virus (SV, 9 HA/ml) for 8 h as indicated. Cell lysates were also immunoblotted with anti-Myc (MAVS) or anti-Flag (NLRP3) antibody (Lower panels, A and B) or anti-Flag (NLRP3) antibody (lower panel, C) as indicated.