Table 2.
Renal function, histopathology and molecular biology parameters in the kidney
| Detected parameters | PostN | PostCS | PostCS+siRNA | P value* | PostTx | PostTx+siRNA | P value* |
|---|---|---|---|---|---|---|---|
| Caspase-3 mRNA |
Not detected |
2.22 ± 0.64 |
0.41 ± 0.12 |
0.017 |
3.77 ± 1.08 |
12.16 ± 3.70 |
0.042 |
| 32 kD caspase-3 |
0.50 ± 0.08 |
2.33 ± 0.28 |
1.11 ± 0.14 |
0.005 |
0.94 ± 0.08 |
0.27 ± 0.04 |
0.002 |
| 17 kD caspase-3 |
0.09 ± 0.01 |
Non-detectable |
Non-detectable |
|
0.07 ± 0.02 |
0.20 ± 0.06 |
0.026 |
| SCr (μmol/L) |
37.5 ± 8.1 |
76.83 ± 9.49 |
80.67 ± 7.10 |
0.753 |
506.4 ± 122.2 |
659.2 ± 49.1 |
0.273 |
| BUN (mmol/L) |
2.8 ± 0.4 |
8.05 ± 1.20 |
8.32 ± 0.95 |
0.865 |
35.9 ± 7.8 |
51.2 ± 6.5 |
0.164 |
| Score of TID/200× field |
Not detected |
9.71 ± 0.56 |
7.93 ± 0.84 |
0.093 |
9.94 ± 0.53 |
11.90 ± 0.21 |
0.011 |
| Active caspase-3+ cells/400× field |
0.01 ± 0.00 |
1.05 ± 0.15 |
0.32 ± 0.10 |
0.004 |
2.96 ± 0.66 |
6.83 ± 1.26 |
0.017 |
| ISEL+ cells /400× field |
0.02 ± 0.01 |
0.30 ± 0.06 |
0.36 ± 0.19 |
0.734 |
1.39 ± 0.29 |
6.06 ± 1.37 |
0.004 |
| MPO+ cells /400× field | 0.77 ± 0.34 | 0.06 ± 0.04 | 0.08 ± 0.05 | 0.921 | 15.92 ± 3.84 | 52.33 ± 13.31 | 0.028 |
PostN, post-nephretomy; PostCS, post cold storage; PostTx, post-transplant; SCr, serum creatinine; BUN, blood urine nitrogen; TID, tubulointerstitial damage; ISEL, in situ end-labeling; MPO, myeloperoxidase. Data were presented as Mean ± SEM. *: The difference calculated between the non-preserved and siRNA-preserved kidney. The caspase-3 mRNA in the non-transplanted (PostN) control kidney was defined as baseline control using a 2−ΔΔ Ct method.