Fig. 2.
Asymmetric binding of H137–211 to the nucleosome. (A) Nucleosome structure, highlighting residues H2A T119 and H3 K37. The DNA structure is taken from the tetra-nucleosome structure with 10 bp DNA at both entry and exit regions (41): H2A is in orange, H3 is in blue, and H4 is in green. (B) Overlay of 1H-15N HSQC spectra of the nucleosome with 15N-labeled H2A in the absence (black) or the presence (orange) of mutant H137–211. (C) Same as in B except the wild-type full-length H11–256 (magenta) was used instead of mutant H137–211. Note that only strong peaks in the spectra are shown (SI Appendix, Fig. S7). In this experiment, the ratio of H137–211 to the nucleosome is 1.0. The dashed lines indicate that the volumes of the peaks in the boxes are integrated together. (D) Ratios of peak volumes of T119, K121, and the region that include K118, E120, and K122 of the H2A tails in the nucleosome in complex with mutant H137–211 to those in the free nucleosome. (E) Same as in D except that the wild-type full-length H11–256 was used instead of mutant H137–211. The asterisks in B–E indicate that the peaks in the dashed box were integrated together when the peak volumes were measured.