. Author manuscript; available in PMC: 2013 Nov 26.

Published in final edited form as: Arch Biochem Biophys. 2008 Mar 14;474(1):10.1016/j.abb.2008.03.008. doi: 10.1016/j.abb.2008.03.008

Table 3.

Substrate specificity of GSH uptake in rat liver mitochondria

Addition	GSH uptake (nmol/min per mg protein)
Addition	− AA	+ AA
Buffer	1.78 ± 0.17	1.51 ± 0.17
Dicarboxylate inhibitors
Butylmalonate	1.47 ±0.39	1.86 ±0.92
Phenylsuccinate	2.67 ± 2.08	3.80 ± 1.56
Butylmalonate + Phenylsuccinate	0.90 ± 0.38^*	0.81 ±0.21^*
Glutamate and aspartate
Glutamate	1.85 ±0.30	1.51 ±0.35
Aspartate	2.51 ± 1.05	1.53 ±0.59
Tricarboxylate substrates
Phosphoenolpyruvate (PEP)	1.84 ±0.90	2.32 ± 1.34
Citrate	1.05 ± 0.23	2.63 ± 1.46
Monocarboxylate substrates
Lactate	1.72 ± 0.65	2.14 ± 0.67
Pyruvate	1.71 ± 0.56	2.01 ± 0.43

Initial rates of uptake of 7.5 mM GSH in suspensions of isolated mitochondria from rat liver were measured with L-[³H-glycyl]-GSH with the indicated inhibitors (each at 15 mM) in both the absence and presence of 2 µM antimycin A (AA). Measurements are means ± SEM of 3 experiments.

P < 0.05 compared with corresponding control value.