. Author manuscript; available in PMC: 2013 Nov 22.

Published in final edited form as: Open Genomics J. 2012;5:10.2174/1875693X01205010018. doi: 10.2174/1875693X01205010018

Table 3. Enrichment Statistics for the P.falciparum Genome Pre- and Post- solution-based Pulldown.

Experiment	Sample ID	Sample Name	Probe Strain	Read Count	% P.falciparum	% Human	% Other
2A	2A i	3D7:human 25:75 prepulldown	~	37261895	14.97	52.88	32.15
	2A ii	3D7:human 25:75 800ng	3D7 1.5μg	36139494	72.44	8.22	19.34
	2A iii	3D7:human 25:75 500ng	3D7 1.5μg	32434631	72.75	7.84	19.41
2B	2B i	3D7:human 25:75 prepulldown	~	40553893	13.42	46.92	39.67
	2B ii	3D7:human 25:75 1600ng	3D7 1.5μg	21241885	66.41	15.90	17.68
	2B iii	3D7:human 25:75 1600ng	3D7 3μg	28703869	68.34	15.34	16.32
	2B iv	3D7:human 25:75 800ng	3D7 1.5μg	41465650	74.77	10.55	14.68
	2B vi	3D7:human 25:75 800ng	3D7 3μg	29455966	73.19	12.19	14.61
2C	2C i	3D7:human 30:70 prepulldown	~	26938319	13.27	46.85	39.88
	2C ii	3D7:human 30:70 55°C hyb	3D7 1.5μg	7909731	67.95	10.29	21.75
	2C iii	3D7:human 30:70 65°C hyb	3D7 1.5μg	8068594	75.64	5.90	18.46
2D	2D i	3D7:human 20:80 prepulldown	~	35442819	11.85	43.56	44.59
	2D ii	3D7:human 20:80	3D7 1.5μg	44006702	74.34	10.21	15.45
	2D iii	3D7:human 20:80 1M TMAC	3D7 1.5μg	38447345	76.82	9.04	14.14
	2D iv	3D7:human 20:80 0.5M TMAC	3D7 1.5μg	44090067	75.20	10.34	14.46
	2D vi	3D7:human 20:80 0.1M TMAC	3D7 1.5μg	44302101	77.08	8.69	14.22
2E	2E i	Dd2:human 30:70 prepulldown	~	41578455	27.06	28.27	44.67
2E	2E ii	Dd2:human 30:70	3D7 1.5μg	33817623	67.49	4.15	28.36
2F	2F i	3D7:human 20:80 prepulldown	~	25542843	9.01	41.08	49.91
2F	2F ii	3D7:human 20:80	3D7, Dd2, IT and HB3	34060230	75.21	7.09	17.69
2G	2G i	Isolate 1:human 20:80 prepulldown	~	45566177	8.88	56.15	34.98
	2G ii	Isolate 2:human 20:80 prepulldown	~	37065625	6.41	51.93	41.66
	2G iii	Isolate 1:human 20:80 mix probe	3D7, Dd2, IT and HB3	36955463	67.19	12.44	20.36
	2G iv	Isolate 2:human 20:80 mix probe	3D7, Dd2, IT and HB3	18331991	65.47	14.89	19.64

Enrichment determined by comparison of Illumina sequencing reads to P.falciparum 3D7 and human reference genomes. Probe amount refers to the amount of DNA used at the start of probe synthesis. Where an amount of sample is listed this is the total amount of DNA added to the hybridisation reaction. Read count is the number of reads obtained per single lane of Illumina GA sequencing. The proportion of the reads mapping to P.falciparum and human genomes are listed. The ‘Other’ category includes all reads that do not map to either the P.falciparum or human genomes and will include primer dimers.