Figure 2. LRG1 promotes angiogenesis.
a, Increased HUVEC tube and branch formation following addition of LRG1 and inhibition by a LRG1 neutralizing antibody. Scale bar 160μm. b, Vessel outgrowth in the metatarsal (top row) and aortic ring (bottom row) assay is enhanced by LRG1 and attenuated by a LRG1 neutralizing antibody. Scale bar = 1,500μm. c, Comparison of vessel growth from metatarsals and aortic rings isolated from WT and Lrg1−/− mice shows reduced angiogenesis in the latter that could be rescued by the addition of LRG1. All images shown are representative and values are expressed as means ± s.e.m of n ≥ 3 independent experimental groups. *P<0.05, **P<0.01, ***P<0.001.