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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: Methods. 2013 Jun 29;64(2):10.1016/j.ymeth.2013.06.019. doi: 10.1016/j.ymeth.2013.06.019

Fig. 2. Optimization of the capture efficiency.

Fig. 2

(A) Cell-capture efficiency of NanoVelcro CTC Chip at flow rates of 0.5, 1, 2, 4, and 8 mL/h. Error bars show standard deviations (n=3–4). CellSearch™ calibration samples containing 100 pre-stained EpCAM-positive SK-BR-3 breast cancer cells were spiked into PBS as a model system. (B) The cell distribution and accumulative cell-capture efficiency in a NanoVelcro CTC Chip were assessed in PBS and normal blood. (C) Cell-capture efficiency at different cell numbers ranging from 10–150 cells mL-1 in two different types of samples: whole blood and PBS buffer. (D) The capture efficiency observed for different prostate cancer cell lines in PBS and blood.