Figure 3. Cell of Origin for murine Brainstem Gliomas.
A. High magnification of a cross section from a four-week old Ntv-a mouse infected with RCAS-GFP. White arrows points to clusters of GFP labeled cells. B. Quantification of the number of GFP-labeled cells at particular locations of a schematic of the cross section of the 4th ventricle. Y-axis denotes the number of GFP-positive cells and the Y-axis denotes to the location of the GFP-positive cells. This cartoon illustrates the cross-section of the 4th ventricle as a triangle. Each side of the triangle (where the floor of the 4th comprises two sides of the triangle) was divided into 4 equal areas and the number of GFP labeled cells were counted in each area, and tabulated. C. High magnification of a cross-section through the 4th ventricle at P2 immunostained with nestin. Black arrows point to positive immunostaining. D. Examples of precursor lesions arising from the 4th ventricle or aqueduct of Ntv-a mice infected with RCAS-PDGF (Bottom left is cross section of aqueduct (AQ)).