FIG. 2.
Comparison of molar carbon fluxes (ovals; values are expressed in millimoles per gram of biomass per hour) and transcript levels of the related enzymes (boxes; values are expressed in arbitrary units) in S. cerevisiae TMB3001 (upper values) and C1 (lower values) during anaerobic chemostat cultivation on 10 g of glucose per liter and 10 g of xylose per liter. The averages ± standard deviations from a DNA microarray analysis of two independent cultures are shown. Independent of the fold change, changes in gene expression were considered significant (indicated by boldface type) upon SAM analysis with an expected median false-positive rate of 1%. The standard deviations for all carbon fluxes were less than 10% of the averages from two independent cultures. Extracellular metabolites are indicated by uppercase letters. Fluxes from the following intracellular metabolites are required for biomass formation but are not shown explicitly: glucose 6-phosphate, ribose 5-phosphate, erythrose 4-phosphate, 3-phosphoglycerate, phosphoenolpyruvate, pyruvate, and acetate (24, 47).