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. 1979 Jan;76(1):377–380. doi: 10.1073/pnas.76.1.377

Monoclonal antibodies reacting with murine teratocarcinoma cells.

P N Goodfellow, J R Levinson, V E Williams 2nd, H O McDevitt
PMCID: PMC382942  PMID: 284353

Abstract

Monoclonal antibodies were produced in vitro by fusing mouse myeloma cells with spleen cells from a rat immunized with the C3H mouse teratocarcinoma C86-S1. After the fusion two clones were chosen for further analysis. The first clone, 3C4-10, produced an antibody recognizing an antigen with a distribution restricted to teratocarcinoma cell lines, an endoderm cell line, and a neuroblastoma. The second clone, 4A1-9, produced an antibody that reacted with all cultured murine cells tested and adult brain. Neither antibody reacted with preimplantation embryos. The 3C4-10 antibody recognized an antigen associated with proteins. The apparent molecular weight of the 3C4-10 antigen was greater than 100,000.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Artzt K., Dubois P., Bennett D., Condamine H., Babinet C., Jacob F. Surface antigens common to mouse cleavage embryos and primitive teratocarcinoma cells in culture. Proc Natl Acad Sci U S A. 1973 Oct;70(10):2988–2992. doi: 10.1073/pnas.70.10.2988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Artzt K., Hamburger L., Jakob H., Jacob F. Embryonic surface antigens: a "quasi-endodermal" teratoma antigen. Dev Biol. 1976 Jul 1;51(1):152–157. doi: 10.1016/0012-1606(76)90130-5. [DOI] [PubMed] [Google Scholar]
  3. Augusti-Tocco G., Sato G. Establishment of functional clonal lines of neurons from mouse neuroblastoma. Proc Natl Acad Sci U S A. 1969 Sep;64(1):311–315. doi: 10.1073/pnas.64.1.311. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Berstine E. G., Hooper M. L., Grandchamp S., Ephrussi B. Alkaline phosphatase activity in mouse teratoma. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3899–3903. doi: 10.1073/pnas.70.12.3899. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dewey M. J., Gearhart J. D., Mintz B. Cell surface antigens of totipotent mouse teratocarcinoma cells grown in vivo: their relation to embryo, adult, and tumor antigens. Dev Biol. 1977 Feb;55(2):359–374. doi: 10.1016/0012-1606(77)90179-8. [DOI] [PubMed] [Google Scholar]
  6. Gachelin G., Kemler R., Kelly F., Jacob F. PCC4, a new cell surface antigen common to multipotential embryonal carcinoma cells, spermatozoa, and mouse early embryos. Dev Biol. 1977 May;57(1):199–209. doi: 10.1016/0012-1606(77)90365-7. [DOI] [PubMed] [Google Scholar]
  7. Galfre G., Howe S. C., Milstein C., Butcher G. W., Howard J. C. Antibodies to major histocompatibility antigens produced by hybrid cell lines. Nature. 1977 Apr 7;266(5602):550–552. doi: 10.1038/266550a0. [DOI] [PubMed] [Google Scholar]
  8. Goldberg E. H., Aoki T., Boyse E. A., Bennett D. Detection of H-2 antigens on mouse spermatozoa by the cytotoxicity test. Nature. 1970 Nov 7;228(5271):570–572. doi: 10.1038/228570a0. [DOI] [PubMed] [Google Scholar]
  9. Gooding L. R., Edidin M. Cell surface antigens of a mouse testicular teratoma. Identification of an antigen physically associated with H-2 antigens on tumor cells. J Exp Med. 1974 Jul 1;140(1):61–78. doi: 10.1084/jem.140.1.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Goridis C., Artzt K., Wortham K. A., Schalchner M. Brain cell surface antigens on embryonal carcinoma cells. Dev Biol. 1978 Jul;65(1):238–243. doi: 10.1016/0012-1606(78)90194-x. [DOI] [PubMed] [Google Scholar]
  11. Hyman R., Stallings V. Complementation patterns of Thy-1 variants and evidence that antigen loss variants "pre-exist" in the parental population. J Natl Cancer Inst. 1974 Feb;52(2):429–436. doi: 10.1093/jnci/52.2.429. [DOI] [PubMed] [Google Scholar]
  12. Jakob H., Boon T., Gaillard J., Nicolas J., Jacob F. Tératocarcinome de la spuris: isolement, culture et propriétés de cellules a potentialités multiples. Ann Microbiol (Paris) 1973 Oct;124(3):269–282. [PubMed] [Google Scholar]
  13. Jensenius J. C., Williams A. F. The binding of anti-immunoglobulin antibodies to rat thymocytes and thoracic duct lymphocytes. Eur J Immunol. 1974 Feb;4(2):91–97. doi: 10.1002/eji.1830040207. [DOI] [PubMed] [Google Scholar]
  14. Kemler R., Babinet C., Eisen H., Jacob F. Surface antigen in early differentiation. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4449–4452. doi: 10.1073/pnas.74.10.4449. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kennett R. H., Denis K. A., Tung A. S., Klinman N. R. Hybrid plasmacytoma production: fusions with adult spleen cells, monoclonal spleen fragments, neonatal spleen cells and human spleen cells. Curr Top Microbiol Immunol. 1978;81:77–91. doi: 10.1007/978-3-642-67448-8_13. [DOI] [PubMed] [Google Scholar]
  16. Knowles B. B., Aden D. P., Solter D. Monoclonal antibody detecting a stage-specific embryonic antigen (SSEA-1) on preimplantation mouse embryos and teratocarcinoma cells. Curr Top Microbiol Immunol. 1978;81:51–53. doi: 10.1007/978-3-642-67448-8_8. [DOI] [PubMed] [Google Scholar]
  17. Koprowski H., Sawicki W., Koldovsky P. Immunologic cross-reactivity between antigen of unfertilized mouse eggs and mouse cells transformed by simian virus 40. J Natl Cancer Inst. 1971 Jun;46(6):1317–1323. [PubMed] [Google Scholar]
  18. Köhler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
  19. Lehman J. M., Speers W. C., Swartzendruber D. E., Pierce G. B. Neoplastic differentiation: characteristics of cell lines derived from a murine teratocarcinoma. J Cell Physiol. 1974 Aug;84(1):13–27. doi: 10.1002/jcp.1040840103. [DOI] [PubMed] [Google Scholar]
  20. Lichtor T., Getz G. S. Cytoplasmic inheritance of rutamycin resistance in mouse fibroblasts. Proc Natl Acad Sci U S A. 1978 Jan;75(1):324–328. doi: 10.1073/pnas.75.1.324. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Martin G. R. Teratocarcinomas as a model system for the study of embryogenesis and neoplasia. Cell. 1975 Jul;5(3):229–243. doi: 10.1016/0092-8674(75)90098-7. [DOI] [PubMed] [Google Scholar]
  22. McBurney M. W. Clonal lines of teratocarcinoma cells in vitro: differentiation and cytogenetic characteristics. J Cell Physiol. 1976 Nov;89(3):441–455. doi: 10.1002/jcp.1040890310. [DOI] [PubMed] [Google Scholar]
  23. Miller R. A., Ruddle F. H. Teratocarcinoma X friend erythroleukemia cell hybrids resemble their pluripotent embryonal carcinoma parent. Dev Biol. 1977 Mar;56(1):157–173. doi: 10.1016/0012-1606(77)90159-2. [DOI] [PubMed] [Google Scholar]
  24. Stern P. L., Willison K. R., Lennox E., Galfrè G., Milstein C., Secher D., Ziegler A. Monoclonal antibodies as probes for differentiation and tumor-associated antigens: a Forssman specificity on teratocarcinoma stem cells. Cell. 1978 Aug;14(4):775–783. doi: 10.1016/0092-8674(78)90333-1. [DOI] [PubMed] [Google Scholar]
  25. Teich N. M., Weiss R. A., Martin G. R., Lowy D. R. Virus infection of murine teratocarcinoma stem cell lines. Cell. 1977 Dec;12(4):973–982. doi: 10.1016/0092-8674(77)90162-3. [DOI] [PubMed] [Google Scholar]
  26. Wiley L. M., Calarco P. G. The effects of anti-embryo sera and their localization on the cell surface during mouse preimplantation development. Dev Biol. 1975 Dec;47(2):407–418. doi: 10.1016/0012-1606(75)90294-8. [DOI] [PubMed] [Google Scholar]

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