FIGURE 5.
Effects of MsDisA and its interaction with MsRadA on bacterial growth and motility. A, the wild type and various recombinant M. smegmatis strains were grown on the surface of solid agar medium (lower panel) and in liquid 7H9 medium (upper panel). Cell aggregation in liquid medium was visualized by Congo red staining. B, determination of growth curves of wild type and recombinant mycobacterial strains. Representative growth curves are shown. C, quantitative real-time PCR assays for the change in expression of MsradA and MsdisA in the recombinant strains. Relative expression levels of the genes were normalized to levels of parB transcripts. Error bars indicate the range of data among three biological replicates. D, Western blotting assays for the protein expression levels in vivo. The strains containing pMV261, pMV261-MSdisA, and pMV261-MSradA-MSdisA plasmids were used in the experiments. Anti-RadA, anti-DisA, and anti-ParB serums were used for the detections. ParB is used as control for the total cell protein. E, effects of MsdisA overexpression and MsdisA-MsradA co-expression on bacterial motility.