Figure 2. Microarray analysis of miR-3134-regulated genes. (A) Unsupervised hierarchial clustering of 220 statistically significant genes (> 1.5-fold, P < = 0.05) in response to miR-3134 overexpression (pc3134) as compared with control (pc) in MCF7 cell line. (A and B) refer to biological replicates. Genes are colored based on absolute expression levels of genes in pc- and pc3134-treated cells. (B) A total of 63% of mir-3134-regulated genes show the presence of at least one AUUUA (pentamer) element (AREscore database). A snapshot of top 20 genes with > eight AUUUA elements in 3′UTR is shown. (C) List of miR-3134-regulated genes showing differential expression in breast cancer based on The Genes-to-Systems Breast Cancer (G2SBC) Database. (D) Pie-chart distribution of significant gene ontology categories and pathways (P value < = 0.05) that were dysregulated by miR-3134-regulated genes.