Figure 3.
CD8+ T cell recognition of antigen presented by alveolar epithelial-derived cells results in peptide-dependent chemokine expression. RNase protection assays were performed on RNA extracted from in vitro cocultures of CD8+ T cells and MLE-Kd cells. Varying doses of antigenic peptide (10–6, 10–8, 10–10, and 10–12 M) were added to the cultures and incubated for 6 hours. With the exception of slight expression of RANTES and MIP-1β, chemokine expression becomes undetectable at 10–12 M peptide.