Fig 2.
DNMT1 is critical for maintaining CpG methylation during early adipogenesis. 3T3-L1 preadipocytes were transfected with control and DNMT1 siRNAs and stimulated to differentiate into adipocytes. (A) Heat map representation of CpG methylation at the Glut4 and Pparg loci at days 0, 2, and 4 after induction of differentiation in siCtrl- or siDNMT1-treated cells. Each row represents one of triplicate samples at each time point, and each column represents one of the 52 or 37 CpGs analyzed, for Glut4 and Pparg, respectively. (B) H3K9me2 ChIP analysis of the Fabp4 and Glut4 genomic loci at day 2 of differentiation. Primers amplified DNA at the indicated distances from the transcriptional start sites. Data were normalized to input and represent triplicate samples (mean ± SD; *, P < 0.05). Data are representative of 2 independent repeats.