Table 1.
Primer sequences, annealing temperatures, and amplification sizes
| Gene | Direction | Primer sequence | Annealing temp (°C) | Amplification size (bp) |
|---|---|---|---|---|
| narG | F | 5′-ACTACGCCGACAACACCAAGTTCGCCGACG-3′ | 68 | 158 |
| R | 5′-AGCGGCGCACATAGTCGACAAAGAACGGAA-3′ | |||
| nirB | F | 5′-GTCCCGGTTCGTTTCCTTCG-3′ | 68 | 155 |
| R | 5′- CGCGGGATACCAATGGACAC-3′ | |||
| glnA | F | 5′-CAACTTCTTTGTGCACGACCCGTT-3′ | 64 | 423 |
| R | 5′-AACTGGTAGTTGATCTCGGCCTGT-3′ | |||
| narK2 | F | 5′-TGCTTCGTGATGCACCCTACTTTCGGCCCA-3′ | 68 | 120 |
| R | 5′-CCGCCGAACACGATCGCGTACAGAAACGAC-3′ | |||
| 16Sa | F | 5′-ATGCATGTCTTGTGGTGGAAAGCG-3′ | 58 | 350 |
| R | 5′-TTCACGAACAACGCGACAAACCAC-3′ |
a
16S gene PCR was done for 25 cycles, while PCRs of other genes were done for 35 cycles.