Fig. 3.
In vivo tumor targeting and antitumor efficacy. (A) In vivo cell internalization. HBSS, the rhodamine-labeled nanopreparation, and its nonsensitive counterpart were injected i.v. in tumor-bearing mice at 5 mg/kg PTX, respectively. At 2 h after injection, tumor and major organs were collected. The cells were dissociated from fresh tissue and analyzed immediately by FACS. (B) Intratumor localization. Tumor sections were stained by Hoechst 33342 and detected by confocal microscopy. (C) Tumor growth inhibition (% of the starting tumor volume). Tumor size was measured every 3 d and calculated as V = lw2/2. *P < 0.05 compared with other groups. (D) Tumor cell apoptosis. Tumor sections were stained by Hoechst 33342, and apoptosis was analyzed by TUNEL assay under confocal microscopy.