Figure 5. Overnight resting does not alter the total number of epitope specific T cells.
(A) Frequencies (left panel) and functionality (right panel) of HIV and CMV specific CD8 T cells were determined by ICS. Data indicate numbers of functional T cells as% of total CD8 T cells. Pie charts show the relative contribution of each functional subpopulation within the total CD8 T-cell response according to their functionality. (B) Frequencies of HIV and CMV specific CD8 T cells determined by multimer staining. Data indicate numbers of multimer-positive T cells as% of total CD8 T cells. (A) and (B): data from five HIV (n = 3) or CMV (n = 2) seropositive individuals are shown; median indicated by black line. (C) Comparison of T cell ICS and multimer staining. Bar charts show frequencies of functional epitope specific CD8 T cells determined by ICS calculated as percentage of corresponding multimer-positive T cells. Representative results of two individuals with known epitope specificities (HLA-B8 restricted HIV Nef and HLA-A2 restricted CMV IE1, respectively) are shown. All analyses were performed on not rested and rested PBMC as indicated.