Figure 6. Expression of membrane-restricted NKG2D ligand MICB.A2 in the TRAMP prostate prevents tumor progression.
(A) Kaplan-Meier plot demonstrating tumor-free survival of TRAMP/MICB.A2 mice. Numbers indicate animals being surveyed. (B) Significantly reduced prostate weight in TRAMP/MICB.A2 bi-Tg mice (n = 21) in comparison with age-matched TRAMP (n = 13) littermates. Age-matched wild-type B6 are shown as normal controls. Numbers of animals are shown in parentheses. (C) Representative images of H&E and p63 IHC staining demonstrate benign prostate histology in TRAMP/MICB.A2 mice. The expression of SV40 Tag in the benign prostate epithelium was also shown. Scale bars: 50 μm. (D) No sMICB was detected in the sera of TRAMP/MICB.A2 mice by ELISA. Asterisk indicates sera from TRAMP/MICB mice as the assay positive control. (E) Blocking NKG2D function with an antibody in TRAMP/MICB.A2 mice resulted in significantly elevated prostate weight. Data in E represent 2 independent experiments.