Figure 4. Synonym mutations of Segment 3 reverse translational repression.
A) eGFP fluorescence detection by laser scanning to evaluate the expression eGFP derived from recombinant constructs that contained the sequence of wild type Nrf2 segment 3 or the mutant with synonym codon substitutions. C) Evaluation of recombinant expression of wild type Nrf2 vs a full length Nrf2 where the sequence corresponding to segment 3 was mutated using the synonym codons described in A. The bar graph to compare the signal intensity of the expression of this two constructs after laser scanning densitometry.