Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Sep 1;27(17):1886–1902. doi: 10.1101/gad.221010.113

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013, Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported), as described at http://creativecommons.org/licenses/by-nc/3.0/.

PMC Copyright notice

Figure 4. — The bromodomains of Bdf2 are required for boundary function. (A) Schematic diagram of Bdf2 protein. The positions of mutations are indicated. (B) Peptide pull-down assays of the indicated recombinant proteins with biotinylated H4 tail (2–24) unmodified and tetra-acetylated at the K5, K8, K12, and K16 (Ac-H4) peptides as well as H3 tail (1–21) unmodified and diacetylated at the K9 and K14 (Ac-H3) peptides. (C) Serial dilution analysis to measure heterochromatin spreading outside of IRC1R∷ura4⁺. (D,E) ChIP analysis of H3K9me2 and Swi6 levels at IRC1R∷ura4⁺ and Bdf2-Flag levels at IRC1R and IRC3L, normalized to act1. The data are averages of three experiments, and error bars represent standard deviation. (F) Western blot analysis of Bdf2-Flag levels. (G) Schematic diagram of the L5-gbs-ura4⁺ reporter. (H) Serial dilution analysis to measure heterochromatin spreading to ura4⁺. All strains used contain epe1Δ to rule out the possibility that Bdf2 recruits Epe1 to establish heterochromatin boundaries. (I) ChIP analysis of H3K9me2 and Swi6 levels at L5-gbs-ura4⁺ and Bdf2-GBD levels at 3xgbs, normalized to act1. The data are averages of three experiments, and error bars represent standard deviation. All strains used contain epe1Δ.