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. 2013 Sep 1;27(17):1886–1902. doi: 10.1101/gad.221010.113

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© 2013, Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported), as described at http://creativecommons.org/licenses/by-nc/3.0/.

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Figure 2. — Bdf2 is enriched at IRCs. (A,C,E) ChIP–chip analysis of Bdf2-Flag and H3K9me2 levels across centromeres I, II, and III. The data are averages of two microarrays. Black arrows in the diagrams indicate the positions of IRCs. Bars indicate tRNA genes. Note that centromere II does not contain IRCs. (B,D,F) ChIP-qPCR analysis of Bdf2-Flag levels at boundary regions, normalized to act1. The positions of amplified regions used for qPCR quantification are indicated. The data are averages of three experiments, and error bars represent standard deviation.