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. 2013 Sep 18;4:287. doi: 10.3389/fimmu.2013.00287

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Copyright © 2013 Trial, Cieslik, Haudek, Duerrschmid and Entman.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Human PBMC were allowed to migrate for the indicated times through human cardiac microvascular endothelium in response to MCP-1 and thereafter adhered to poly d-lysine coated coverslips. The cells were stained for the external markers CD86, CD206, and CD301, and after fixation and permeabilization, for the internal markers NOS2 and prolylhydroxylase (labeled prolylhyase in the figure). DAPI was used to stain cell nuclei blue. The insets are cells at either higher magnification (CD301) or cells with external staining and an internal marker (CD86 plus NOS2 and CD206 plus prolylhydroxylase) with white arrows extending from the label. The white arrow within the center panel indicates a CD86 positive cell in the low magnification field, and the inset at top right shows a cell that is double positive for CD86 and CD206. White bars represent 20 μm.